10213 strain Search Results


10213 strain  (ATCC)
94
ATCC 10213 strain
10213 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polymyxin resistant a baumannii  (ATCC)
97
ATCC polymyxin resistant a baumannii
The MIC and MBC of reagents against <t> A. baumannii </t> strains (mg/L).
Polymyxin Resistant A Baumannii, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
polymyxin resistant a baumannii - by Bioz Stars, 2026-10
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Image Search Results


The MIC and MBC of reagents against A. baumannii strains (mg/L).

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter baumannii to polymyxins

doi: 10.3389/fcimb.2024.1467607

Figure Lengend Snippet: The MIC and MBC of reagents against A. baumannii strains (mg/L).

Article Snippet: The A. baumannii strains used in this study were listed in , among which the type strain ATCC 19606 and 17978 are polymyxin-susceptible, six clinical strains were isolated from Qilu Hospital of Shandong University and identified as polymyxin-resistant A. baumannii isolates, and 10213 was obtained by ATCC 19606.

Techniques:

Activities of crude extracts from various Aloe species against A. baumannii COLR 10213 and component analysis of extracts from A. arborescens and A. barbadensis . (A) Representative inhibition zones against polymyxin-resistant A. baumannii COLR 10213 produced by the crude extracts from various Aloe species obtained using different solvent systems. The first panel shows the results of the respective solvent and 10 mg/L CST solution as controls. (B) Diameters of inhibition zones (mm) produced by the crude extracts from aloes in triplicate experiments (mean ± standard deviation, n = 3). (C) HPLC fingerprint chromatograms of crude extracts from A. arborescens (AA) using different solvent systems, with peak alignment at 254 nm wavelength. Chromatographic peaks of aloe emodin, rhein, emodin, and aloesin serve as standards for comparing retention times. (D) HPLC fingerprint chromatograms of crude extracts from A. barbadensis (AB). (E) High-resolution mass spectrometry analysis of indicated peaks (denoted as peaks 1-4 in panel C, D ) in crude extracts of AA and AB using the Lac-Glu solvent. AA, A. arborescens ; AB, A. barbadensis ; AF, A. ferox ; AL, A. vera L. var. chinensis (Haw.) Berger; MeOH, methanol; ChClGlu, choline chloride/D-glucose in a molar ratio of 1:1; ChClMa, choline chloride/malic acid in a molar ratio of 2:1; ChClCa, choline chloride/citric acid in a molar ratio of 2:1; LacGlu, lactic acid/D-glucose in a molar ratio of 5:1.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter baumannii to polymyxins

doi: 10.3389/fcimb.2024.1467607

Figure Lengend Snippet: Activities of crude extracts from various Aloe species against A. baumannii COLR 10213 and component analysis of extracts from A. arborescens and A. barbadensis . (A) Representative inhibition zones against polymyxin-resistant A. baumannii COLR 10213 produced by the crude extracts from various Aloe species obtained using different solvent systems. The first panel shows the results of the respective solvent and 10 mg/L CST solution as controls. (B) Diameters of inhibition zones (mm) produced by the crude extracts from aloes in triplicate experiments (mean ± standard deviation, n = 3). (C) HPLC fingerprint chromatograms of crude extracts from A. arborescens (AA) using different solvent systems, with peak alignment at 254 nm wavelength. Chromatographic peaks of aloe emodin, rhein, emodin, and aloesin serve as standards for comparing retention times. (D) HPLC fingerprint chromatograms of crude extracts from A. barbadensis (AB). (E) High-resolution mass spectrometry analysis of indicated peaks (denoted as peaks 1-4 in panel C, D ) in crude extracts of AA and AB using the Lac-Glu solvent. AA, A. arborescens ; AB, A. barbadensis ; AF, A. ferox ; AL, A. vera L. var. chinensis (Haw.) Berger; MeOH, methanol; ChClGlu, choline chloride/D-glucose in a molar ratio of 1:1; ChClMa, choline chloride/malic acid in a molar ratio of 2:1; ChClCa, choline chloride/citric acid in a molar ratio of 2:1; LacGlu, lactic acid/D-glucose in a molar ratio of 5:1.

Article Snippet: The A. baumannii strains used in this study were listed in , among which the type strain ATCC 19606 and 17978 are polymyxin-susceptible, six clinical strains were isolated from Qilu Hospital of Shandong University and identified as polymyxin-resistant A. baumannii isolates, and 10213 was obtained by ATCC 19606.

Techniques: Inhibition, Produced, Solvent, Standard Deviation, Mass Spectrometry

Antibacterial and bactericidal activity of combination treatment against polymyxin-resistant A. baumannii strains. (A) MICs (mg/L) against A. baumannii strains by PMB and CST combined with E, AE, R, EAR, or CE, without EDTA (left panel) and with 256 mg/L EDTA supplementation (right panel). The values in parentheses represent optimal concentrations of E, AE, R, EAR, and CE (mg/L). The color legend represents the calculated FICI based on the MIC values provided in <xref ref-type= Table 1 . FICI < 0.5 indicates synergism, 0.5 ≤ FICI < 1 indicates additivity. (B) MBCs (mg/L) against A. baumannii strains by PMB and CST combined with E, AE, R, EAR, or CE, without EDTA (left panel) and with 256 mg/L EDTA supplementation (right panel). The values in parentheses represent optimal concentrations of E, AE, R, EAR, and CE (mg/L). The color legend corresponds to varying levels of drug sensitivities. E, emodin; AE, aloe emodin; R, rhein; EAR, emodin/aloe emodin/rhein in a mass ratio of 1:1:1; CE, crude extract of A. barbadensis by Lac-Glu solvent; PMB, polymyxin B; CST, colistin; FICI, fractional inhibitory concentration index. " width="100%" height="100%">

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter baumannii to polymyxins

doi: 10.3389/fcimb.2024.1467607

Figure Lengend Snippet: Antibacterial and bactericidal activity of combination treatment against polymyxin-resistant A. baumannii strains. (A) MICs (mg/L) against A. baumannii strains by PMB and CST combined with E, AE, R, EAR, or CE, without EDTA (left panel) and with 256 mg/L EDTA supplementation (right panel). The values in parentheses represent optimal concentrations of E, AE, R, EAR, and CE (mg/L). The color legend represents the calculated FICI based on the MIC values provided in Table 1 . FICI < 0.5 indicates synergism, 0.5 ≤ FICI < 1 indicates additivity. (B) MBCs (mg/L) against A. baumannii strains by PMB and CST combined with E, AE, R, EAR, or CE, without EDTA (left panel) and with 256 mg/L EDTA supplementation (right panel). The values in parentheses represent optimal concentrations of E, AE, R, EAR, and CE (mg/L). The color legend corresponds to varying levels of drug sensitivities. E, emodin; AE, aloe emodin; R, rhein; EAR, emodin/aloe emodin/rhein in a mass ratio of 1:1:1; CE, crude extract of A. barbadensis by Lac-Glu solvent; PMB, polymyxin B; CST, colistin; FICI, fractional inhibitory concentration index.

Article Snippet: The A. baumannii strains used in this study were listed in , among which the type strain ATCC 19606 and 17978 are polymyxin-susceptible, six clinical strains were isolated from Qilu Hospital of Shandong University and identified as polymyxin-resistant A. baumannii isolates, and 10213 was obtained by ATCC 19606.

Techniques: Activity Assay, Solvent, Concentration Assay

Time-kill curves of different drug combinations against polymyxin-resistant A. baumannii strains. (A) Time-kill curves of PMB (2 mg/L, middle panel) and CST (2 mg/L, right panel) combined with EDTA (256 mg/L) and EAR (ranging from 8 to 32 mg/L) against A. baumannii 1415E. PMB, CST, and EDTA are used alone or in combinations as controls (left panel). (B) Time-kill curves of PMB (2 mg/L) and CST (2 mg/L) combined with EDTA (256 mg/L) and EAR (32 mg/L) against six polymyxin-resistant strains of A. baumannii . PMB and CST are used as controls. Data are presented as mean ± standard deviation (n = 3). EAR, emodin/aloe emodin/rhein in a mass ratio of 1:1:1; PMB, polymyxin B; CST, colistin.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter baumannii to polymyxins

doi: 10.3389/fcimb.2024.1467607

Figure Lengend Snippet: Time-kill curves of different drug combinations against polymyxin-resistant A. baumannii strains. (A) Time-kill curves of PMB (2 mg/L, middle panel) and CST (2 mg/L, right panel) combined with EDTA (256 mg/L) and EAR (ranging from 8 to 32 mg/L) against A. baumannii 1415E. PMB, CST, and EDTA are used alone or in combinations as controls (left panel). (B) Time-kill curves of PMB (2 mg/L) and CST (2 mg/L) combined with EDTA (256 mg/L) and EAR (32 mg/L) against six polymyxin-resistant strains of A. baumannii . PMB and CST are used as controls. Data are presented as mean ± standard deviation (n = 3). EAR, emodin/aloe emodin/rhein in a mass ratio of 1:1:1; PMB, polymyxin B; CST, colistin.

Article Snippet: The A. baumannii strains used in this study were listed in , among which the type strain ATCC 19606 and 17978 are polymyxin-susceptible, six clinical strains were isolated from Qilu Hospital of Shandong University and identified as polymyxin-resistant A. baumannii isolates, and 10213 was obtained by ATCC 19606.

Techniques: Standard Deviation

In vitro assessment of resistant mutation frequency of polymyxin B and colistin alone or in combination with EDTA and EAR against polymyxins-sensitive strains of A. baumannii ATCC 19606 and 17978. Box plots represent the resistant mutation frequency of ATCC 19606 (first panel) and 17978 (second panel) to PMB (A) and CST (B) after exposure to 10 mg/L polymyxins alone or supplemented with EDTA (256 mg/L) and EAR (ranging from 0 to 500 mg/L). Each dot represents the value of an individual measurement (n = 5), and the mean value is shown by the line. EAR, emodin/aloe emodin/rhein mass ratios of 1:1:1; PMB, polymyxin B; CST, colistin. **, P < 0.01, ***, P < 0.001; ND, not detected; NS, not significant.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter baumannii to polymyxins

doi: 10.3389/fcimb.2024.1467607

Figure Lengend Snippet: In vitro assessment of resistant mutation frequency of polymyxin B and colistin alone or in combination with EDTA and EAR against polymyxins-sensitive strains of A. baumannii ATCC 19606 and 17978. Box plots represent the resistant mutation frequency of ATCC 19606 (first panel) and 17978 (second panel) to PMB (A) and CST (B) after exposure to 10 mg/L polymyxins alone or supplemented with EDTA (256 mg/L) and EAR (ranging from 0 to 500 mg/L). Each dot represents the value of an individual measurement (n = 5), and the mean value is shown by the line. EAR, emodin/aloe emodin/rhein mass ratios of 1:1:1; PMB, polymyxin B; CST, colistin. **, P < 0.01, ***, P < 0.001; ND, not detected; NS, not significant.

Article Snippet: The A. baumannii strains used in this study were listed in , among which the type strain ATCC 19606 and 17978 are polymyxin-susceptible, six clinical strains were isolated from Qilu Hospital of Shandong University and identified as polymyxin-resistant A. baumannii isolates, and 10213 was obtained by ATCC 19606.

Techniques: In Vitro, Mutagenesis

Therapeutic potential of combination therapy for polymyxin-resistant A. baumannii 1415E induced skin infections in BALB/C mice. (A) The bacterial burden of A. baumannii 1415E in infected wounds by counting CFUs at the 0 th , 5 th , and 6 th d post-infection (n = 5). (B) Representative photographs of the infected wounds on mice skin captured at the 1 st , 3 rd , 4 th , 6 th , 8 th , 10 th , and 12 th d post-treatments with PBS buffer, PMB (2 mg/mL) or drug combination (32 mg/kg EDTA+ 4 mg/kg EAR+ 2 mg/kg PMB). Scale bar represents 2 mm. (C) Box plots represent the measured wound areas (mm 2 ) at the 1 st , 3 rd , 4 th , 6 th , 8 th , 10 th , and 12 th d post-treatments (n = 6). (D) The bacterial burden of A. baumannii 1415E in infected wounds by CFU count at 12 days post-treatments (n = 6). The group treated with PBS buffer serves as the mock control. Each dot represents the value of an individual measurement, and the mean value is shown by the line. *, P < 0.05, **, P < 0.01, ***, P < 0.001. EAR, emodin/aloe emodin/rhein in a mass ratio of 1:1:1; PMB, polymyxin B; NS, not significant.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter baumannii to polymyxins

doi: 10.3389/fcimb.2024.1467607

Figure Lengend Snippet: Therapeutic potential of combination therapy for polymyxin-resistant A. baumannii 1415E induced skin infections in BALB/C mice. (A) The bacterial burden of A. baumannii 1415E in infected wounds by counting CFUs at the 0 th , 5 th , and 6 th d post-infection (n = 5). (B) Representative photographs of the infected wounds on mice skin captured at the 1 st , 3 rd , 4 th , 6 th , 8 th , 10 th , and 12 th d post-treatments with PBS buffer, PMB (2 mg/mL) or drug combination (32 mg/kg EDTA+ 4 mg/kg EAR+ 2 mg/kg PMB). Scale bar represents 2 mm. (C) Box plots represent the measured wound areas (mm 2 ) at the 1 st , 3 rd , 4 th , 6 th , 8 th , 10 th , and 12 th d post-treatments (n = 6). (D) The bacterial burden of A. baumannii 1415E in infected wounds by CFU count at 12 days post-treatments (n = 6). The group treated with PBS buffer serves as the mock control. Each dot represents the value of an individual measurement, and the mean value is shown by the line. *, P < 0.05, **, P < 0.01, ***, P < 0.001. EAR, emodin/aloe emodin/rhein in a mass ratio of 1:1:1; PMB, polymyxin B; NS, not significant.

Article Snippet: The A. baumannii strains used in this study were listed in , among which the type strain ATCC 19606 and 17978 are polymyxin-susceptible, six clinical strains were isolated from Qilu Hospital of Shandong University and identified as polymyxin-resistant A. baumannii isolates, and 10213 was obtained by ATCC 19606.

Techniques: Infection, Control